Biodegradation of Organochlorine Pesticides by Means of Pectobacterıum Wasabiae

Abstract

Water pollution is a major cause of global concern as it leads to the onset of numerous fatal diseases which are responsible for the death of 14000 people. The Aquatic systems contamination by pesticides is one of the most serious problems that might face the environment and organisms as a whole. One of the safest potential techniques in the remediation of chemically contaminated water is the biological remediation. Bioremediation is one of the friendliest environmental and cost-effective methods for the decontamination and detoxification of pesticides contaminated water. From this regard, this work presents the investigation of the biodegradation potential of 17 tested organochlorine pesticides by some bacterial isolates. Moreover, the remaining toxicity of the 17 tested organochlorine pesticides with tested strains was evaluated to confirm the complete removal of any toxic materials. The testing of the isolated bacteria for the biodegradation capability showed that all the isolated microorganisms having remarkable variation in the biodegradation process which could be detected in the aqueous media containing the active ingredients of the 17 OCPs by two approaches, the analysis of the OCP residues at the end of the incubation period and measuring the bacterial growth in terms of the total viable count and the optical density OD. The bacterial isolate O3 presented the great degradation value as well as high total viable count and OD measurements. According to the morphological, biochemical and 16S RNA, the bacterial isolate was identified as Pectobacterium wasabiae CFBP 3304. There is no remaining toxicity of the 17 OCPs detected in aqueous media previously treated with Anabaena sp. The study findings strongly support that bioremediation by Pectobacterium wasabiae CFBP 3304 to be a promising approach for the biodegradation and removal of OCPs residues in an aqueous system.