article · ACS Applied Nano Materials
Bimetallic cobalt molybdate (CoMoO4) nanorods with strong peroxidase activity were synthesised using a hydrothermal method followed by calcination. These nanorods were applied as chemiluminescence catalysts for the first time, increasing the light intensity of a luminol and hydrogen peroxide system by nearly 750-fold. Taking advantage of the quenching effect that dopamine exerts on this chemiluminescence reaction, a sensitive and rapid detection platform was developed. The analytical method achieved a linear range of 10 to 500 nanomolar and a limit of detection of 1.98 nanomolar. Testing on serum samples using a standard addition approach confirmed the platform's reliability, yielding recovery rates between 97.2% and 104.3%. This work demonstrates the utility of transition-metal nanozymes for sensitive optical biosensing.
Dopamine is an essential biomarker, and measuring it reliably requires sensitive and rapid detection methods. By significantly boosting light emission in a standard chemical system, these stable, biocompatible nanozymes offer an accessible way to detect dopamine at trace concentrations. Demonstrating success in serum samples shows that this approach holds promise for clinical testing and biochemical diagnostics.
The method could enable rapid diagnostic sensors for biomedical laboratories and clinical researchers tracking dopamine. At present, the technology is an applied and tested laboratory assay, demonstrated on serum samples using standard additions. Commercial translation would require developing the assay into a standardised, user-friendly testing kit or integrating it with portable chemiluminescence instruments, alongside extensive clinical validation against current diagnostic benchmarks.
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Transition-metal-based nanozymes show great potential for chemiluminescence (CL) detection due to their stability, excellent catalytic properties, good biocompatibility, and facile synthesis. In this study, bimetallic CoMoO4 nanorods with high peroxidase activity were synthesized using a hydrothermal method, followed by calcination treatment. The as-synthesized CoMoO4 nanorods were utilized as catalysts for CL for the first time. They enhance the CL intensity of the luminol/hydrogen peroxide system by nearly 750-fold. Furthermore, a sensitive and rapid platform for dopamine detection was established based on the quenching effect of dopamine on the CL signal of this system. The proposed CL method for dopamine exhibited a linear range of 10–500 nM and a detection limit of 1.98 nM. Moreover, dopamine in serum samples was successfully measured using a standard addition method, showing a satisfactory recovery of 97.2–104.3%. This work provides robust support for the development of efficient and sensitive platforms for CL detection.
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DOI: 10.1021/acsanm.3c05309
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