Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls

Abstract

This study aimed to identify the single nucleotide polymorphisms (SNPs) in a partial sequence of the leptin gene and its potential association with the semen quality parameters and mRNA expression levels of the leptin gene in the Egyptian buffalo' bulls. A total of 20 Egyptian buffalo bulls were used in this study. The selected amplicon of the leptin gene was amplified using a specific primer. After that, the SNPs detection and genotyping were performed based on sequence analysis and high-resolution melting (HRM)-PCR. A c.216A>G synonymous SNP (g.14328A>G) was identified in the assessed partial leptin sequence. This SNP showed significant linkages with the semen profile. In this regard, the heterozygous AG genotype exhibited significantly higher progressive motility (PR), sperm concentration, total sperm count, and higher LH and testosterone hormone levels. While the GG genotype showed higher normal morphology form % and functional sperm %. Moreover, AG and GG genotypes displayed significant upregulation of leptin mRNA levels compared to the AA genotype. The g.14328A>G SNP could be considered an effective marker in marker-assisted selection and breeding programs for improving the fertility of Egyptian buffalo bulls.