Assessment of Oxidative Stress Biomarkers as Indicators of Environmental Pollution in Water Hyacinth and Nile Tilapia from Effluent-Impacted Wupa River Abuja

Abstract

The danger to environmental productivity posed by many human activities is making water contamination an essential problem. Assessing oxidative stress biomarkers as markers of environmental contamination in water hyacinth and Nile tilapia from the Wupa River in Abuja that is damaged by effluent is the goal of this study. Samples were gathered and examined according to established protocols. Biomarker result shows hydrogen peroxide activities in the leaves of water Hyacinth (101.76 ±11.70-104.17 ± 17.78 mol/L) stem (81.22 ± 9.86-87.68 ± 10.31 mol/L). Superoxide anion of the leaves (25.33 ± 3.56-28.06 ± 6.09 mol/m 3 ), stem (24.51 ± 2.99 - 26.16 ±1.84 mol/m 3 ). Catalase enzymatic activities (43.57±2.12 - 48.23 ± 19.06), stem (39.73±4.95-47.12 ± 5.00). The Malondialdehyde of the leaves (348.04 ± 220.43-455.08 ± 137.99 g/mol) and the stem (180.08 ± 151.08-252.04 ±147.67 g/mol). In the Fish, Hydrogen peroxide (H₂O₂) levels were highest in the liver, ranging from 363.62 ± 46.16 mol/L (upstream) to 362.81 ± 225.78 mol/L (downstream), significantly exceeding those in the heart and gills. Superoxide anion concentrations followed a similar pattern, with liver values ranging from 41.03 ± 8.75 mol/m³ (downstream) to 82.61 ± 64.88 mol/m³ (upstream), again surpassing levels in the heart and gills. Catalase activity was relatively stable across tissues, with values in the liver ranging from 33.75 ± 5.49 (upstream) to 38.51 ± 2.04 (downstream). Malondialdehyde (MDA) levels, an indicator of lipid peroxidation, were significantly elevated in the gills, particularly upstream (30.89 ± 10.87 g/mol), when compared to liver and heart values. The Biomarker results demonstrate that water hyacinth and Nile Tilapia can be effectively used to assess the pollution status of the Wupa River, with the biomarkers such hydrogen peroxide, catalase and superoxide anion levels reflecting the organism’s response to environmental stressors.