article · Bionatura
Tissue culture is a potent means for producing clonally propagated plants. However, genetically identical regenerants are of great concern due to their economic consequences. Therefore, polymerase chain reaction (PCR)-based molecular markers are employed to detect somaclonal variations. In this study, the genetic fidelity of in vitro raised Philodendron bipinnatifidum clones, and their mother plant was tested using 11 randomly amplified polymorphic DNA (RAPD) markers. The RAPD decamers produced 92 amplicons with 8.4 bands ranging from 260-5000 bp. The bands varied from one to fifteen for primer 8 and primer 7, respectively. The genetic similarity between the micro propagated plantlets and mother plant of P. bipinnatifidum was nearly 100%, assuring uniformity and true-to-type regenerated plantlets for this commercially crucial ornamental plant.
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DOI: 10.21931/rb/2022.07.01.10
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