Assessment of DNA ploidy on experimentally induced hamster buccal pouch carcinoma (Histological and flow cytometric studies)

Abstract

The current study's target is directed to assessment of DNA ploidy on experimentally induced hamster buccal pouch carcinoma. Material and methods: five weeks old thirty male Syrian hamsters, weighing 80-120g. Three distinct groups of experimental animals were used (10 in each). GI (negative control): animals left untreated. GII (DMBA): animals were painted with 0.5 percent 7, 12-dimethylbenz (a) anthracene (DMBA) (3times/week/14 weeks). GIII (Paclitaxel- metformin): following DMBA application, animals injected intraperitoneally (IP) with paclitaxel (IP) (20mg/kg/ twice a week /3 weeks) and metformin (IP) (10 mg/kg/ 3 a week /3 weeks). After euthanization, all right pouches were surgically excised, bisected with one section fixed and processed for H&E and other section of the fresh tissue were used for flow cytometric (FCM) assessment. Results: Gross observation and histopathological changes were recorded. By FCM, the data exhibited that in GII DNA diploid was detected in (20%) of cases and DNA aneuploidy was detected in (80%) of cases while in GIII DNA diploid was detected in (51.3%) of cases. While DNA aneuploidy was detected in (48.7%) of cases. The SPF values of the diploid lesions in GII and GIII, ranged between (1.93% and 25.94%, 13.40%and 42.30%,) respectively. The SPF values of the aneuploid lesions in GII and GIII ranged between (10.25% and 51.50%, 5.50% and 42.50%,) respectively.Conclusion: There is a substantial association between tumor DNA ploidy or SPF and responsiveness to metformin in combination with standard chemotherapeutic drugs.Keywords: HBP carcinoma, Paclitaxel, DNA ploidy.