Antibiotics Evaluation of Bacterial Isolates Associated with Imo State University Microbiology Laboratory and Sub-Offices

Abstract

An analysis was carried out to determine the antibiotics evaluation of bacterial isolates associated with Imo State University (IMSU) Microbiology laboratory and sub offices. Swab sticks used for streaking were inoculated unto a freshly prepared nutrient agar, MacConkey agar for bacteria. Identification of bacteria was performed by determining their gram staining properties, biochemical reactions and sugar tests indicating their presence. The microbial isolates obtained from this study were identified to belong to bacteria genus of Klebsiella sp., Escherichia coli, Bacillus sp., Streptococcus sp., Staphylococcus sp., Shigella sp., Micrococcus sp., and Pseudomonas sp. Klebsiella sp. had the highest percentage of occurrence (39.3%) while Pseudomonas sp had the least occurrence (1.8%). Bacteria isolates showed the highest percentage of susceptibility to Tarivid (OFX) 21(25%) followed by Augmentin (AU) 13(15.5%), Gentamycin (CN) 10(11.9%), Nalidixic (NA) 9(10.7%), Ceporex (CEP) 7(8.3%), Septrin (SXT) 4(4.8%), Ciprofloxacin (CPX) 3(3.6%) while the lowest percentage of susceptibility was to Streptomycin (S) 1(1.2%). The effectiveness of decontamination ranges from high-level sterilization to simple cleaning with soap and water. Levels of decontamination include Sterilization, Disinfection, Antisepsis, and Cleaning. It is recommended that ethylene oxide gas is used in Sterilization of the air before any analysis is carried out.