article · Molecules
Nanofibrous wound bandages were developed using a blend of honey, tripolyphosphate, and chitosan, loaded with gold nanoparticles, capsaicin extracted from hot pepper, or a combination of both agents. The resulting nanofibre mats were assessed in laboratory tests against multiple bacterial strains, including Pasteurella multocida, Klebsiella rhinoscleromatis, Staphylococcus pyogenes, and Vibrio vulnificus, in comparison with standard antibiotics. Cell viability was evaluated through an MTT assay, while healing efficacy was examined using an in vivo wound-closure model in rabbits. Bandages loaded separately with capsaicin or gold nanoparticles showed significant antibacterial activity that surpassed controls and standard antibiotics. In addition, the mats boosted cell proliferation and accelerated wound-closure rates, offering an effective biomaterial platform for topical healing.
Treating skin injuries requires dressings that simultaneously protect against microbial infection and encourage tissue repair. By integrating natural bioactive compounds such as honey and capsaicin with chitosan and gold nanoparticles, these dressings offer strong antibacterial protection alongside tissue regeneration. This demonstrates an effective route towards advanced topical wound treatments that reduce infection risks and shorten recovery times.
The primary application is in advanced wound care and topical skin dressings, aimed at wound-care product manufacturers and healthcare systems. The technology has been applied and tested in both laboratory and in vivo animal models, situating it at a pre-clinical stage of development. Commercialisation would require further clinical safety and efficacy trials, alongside the development of scalable manufacturing processes for the electrospun mats.
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This paper describes the preparation, characterization, and evaluation of honey/tripolyphosphate (TPP)/chitosan (HTCs) nanofibers loaded with capsaicin derived from the natural extract of hot pepper (<i>Capsicum annuum</i><i>L</i>.) and loaded with gold nanoparticles (AuNPs) as biocompatible antimicrobial nanofibrous wound bandages in topical skin treatments. The capsaicin and AuNPs were packed within HTCs in HTCs-capsaicin, HTCs-AuNP, and HTCs-AuNPs/capsaicin nanofibrous mats. In vitro antibacterial testing against <i>Pasteurella multocida, Klebsiella rhinoscleromatis,</i><i>Staphylococcus pyogenes,</i> and <i>Vibrio vulnificus</i> was conducted in comparison with difloxacin and chloramphenicol antibiotics. Cell viability and proliferation of the developed nanofibers were evaluated using an MTT assay. Finally, in vivo study of the wound-closure process was performed on New Zealand white rabbits. The results indicate that HTCs-capsaicin and HTCs-AuNPs are suitable in inhibiting bacterial growth compared with HTCs and HTCs-capsaicin/AuNP nanofibers and antibiotics (<i>P</i> < 0.01). The MTT assay demonstrates that the nanofibrous mats increased cell proliferation compared with the untreated control (<i>P</i> < 0.01). In vivo results show that the developed mats enhanced the wound-closure rate more effectively than the control samples. The novel nanofibrous wound dressings provide a relatively rapid and efficacious wound-healing ability, making the obtained nanofibers promising candidates for the development of improved bandage materials.
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DOI: 10.3390/molecules25204770
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