Anti-<i>Candida</i> cross-streak activity by actinomycetes, set 1: ActinoData project

Abstract

Photographs taken of cross-streak agar plates with an antibiotic-producing actinomycete strain streaked in the middle of the plate, incubated for 7 days at 30 degrees Celsius (optimal growth temperature of actinomycetes), and the yeast test strains (pathogens) streaked from the outer edge of the agar plate up to the actinomycete growth in the middle. Plates are incubated at 30 degrees Celsius (optimal growth temperature of the pathogenic strains) for 24hrs and inspected for activity. Gaps between the yeast streak and the central growth streak serve as an indicator that the actinomycete produced a bioactive compound that inhibited the growth of the bacterium. The yeast strains (pathogens) streaked on the plate are in the following order:On the left, top to bottom:<i>Candida kruseii</i> ATCC 34135, and <i>Candida krusei</i> ATCC 14243On the right, top to bottom:<i>Candida tropicalis</i> ATCC 750, and <i>Candida tropicalis</i> ATCC 13803<br><br>Images are labelled as follow:actinomycete strain name_media type_Candida_30C_agar<br>The actinomycete strain name will vary, while the media type could be M19 (g/L: 20 mannitol, 10 casamino acids, 20 peptone, 20 bacteriological agar, pH7.0) or ISP2 (g/L: 10 malt extract, 4 yeast extract, 4 glucose, 20 bacteriological agar, pH7.0). 'Candida_30C' denotes that the analysis was performed on yeast test strains that have an optimal growth temperature of 30 degrees Celsius, while 'agar' refers to the side of the plate visualized in the photograph.<br>