Ameliorative Effects of 5-7, Dihydroxy Flavone (Chrysin) on Hippocampus of Wistar Rats with Doxorubicin-induced Cognitive Impairment

Abstract

Concern is increasing regarding the effect of chemotherapy induced cognitive impairment on oncology patients. This sought to investigate the effect of 5-7, dihydroxyflavone on the hippocampus of wistar rats with Doxorubicin induced cognitive impairment. 30 male Wistar rats were procured and acclimatized for 14 days with feed and water, they were divided into six (6) experimental groups of five (5) animals each. Group 1 served as normal control. Group 2 was induced with 2mg/kg of DOX and was untreated. Group 3 received 150mg/kg of Chrysin. Groups 4, 5 and 6 were induced with 2mg/kg of doxorubicin and treated with Chrysin at doses of 50mg/kg, 100mg/kg, and 150mg/kg respectively. The study lasted for 21 days. The body weight of the animals were recorded three (3) times before and after induction and then recorded again at the end of the 21 days treatment. At the end of the experiment, there were significantly increase in body weight. The increase was more pronounce in group 1 and group 2. In the oxidative stress analysis and ELISA analysis on the levels of inflammatory cytokines (IL-1 & IL-6), there was a significant increase in the levels compared to the control group 1. In the neurobehavioural test, group 6 had a high spontaneous alternation percentage compared to other groups. The treatment with Chrysin significantly had an ameliorative effect on the treated animal groups and in group 3. The result from this work suggest that Chrysin extract had an ameliorative effect on cognitive impairment of the hippocampus.