Additional file 1 of Contrasting patterns of trematode and host snail dispersal across clusters of tropical crater lakes

Abstract

Supplementary material 1: Table S1: Infection prevalence (in %) of the three parasites in the Bulinus tropicus populations analysed. Sample sizes (n) represent the number of host snails screened for infection per lake. Table S2: GenBank accession numbers of the sequences used in the study. Table S3: Sample size (n), number of unique haplotypes (Nh), number of polymorphic sites (Np), haplotype diversity (h) and nucleotide diversity (π) with standard deviation in the various populations of Bulinus tropicus, Petasiger sp., Echinoparyphium sp. and Plagiorchioidea sp. Table S4: P-values obtained from the pairwise Wilcoxon tests with Hommel’s corrections to compare haplotype and nucleotide diversity between analysed markers for each species. Populations of Plagiorchioidea sp. were not included as no genetic polymorphism was observed within or among these populations. Populations with sample size < 5 are excluded from these analyses. Values in bold indicate significant differences. Cells containing – indicate that no comparison was performed because the markers could not be amplified. Table S5: P-values obtained from the pairwise Wilcoxon tests with Hommel’s corrections to compare haplotype and nucleotide diversity between the species analysed for each marker sequenced. Populations of Plagiorchioidea sp. were not included as no genetic polymorphism was observed within or among these populations. Populations with sample size < 5 are excluded from these analyses. Values in bold indicate significant differences. Table S6: Tajima’s D and Fu’s Fs tests of neutral evolution. Lakes with low sample size (< 5 are excluded) and lakes with only 1 haplotype are indicated with –. Significance of p-value is signalled by * if < 0.05 for Tajima’s D or < 0.02 for Fu’s Fs and ** if < 0.001 (both tests). Table S7: Results of Mantel tests to examine correlations between Slatkin’s genetic distance among populations and the Euclidian geographic distance between the lakes where these populations were sampled. Figure S1: TCS haplotype networks of: A. COI1 marker of Bulinus tropicus (n = 823); B. COI2 marker of B. tropicus (n = 812); C. NAD1 marker of B. tropicus (n = 816); D. COI1 of Petasiger sp. (n = 71); ); E. COI2 of Petasiger sp. (n = 171); ); F. cytb of Petasiger sp. (n = 128); G. cytb of Echinoparyphium sp. (n = 116). Each circle represents a unique haplotype, circle size being proportional to the number of specimens with the haplotype/ Colors proportions in the circles provides information on the population of the specimens for each population (blue colors = populations from the Bunyaruguru cluster, red to yellow colors = populations from the Ndali-Kasenda cluster). Dashes on segments connecting haplotype circles represent the number of inferred mutation steps between haplotypes. Figure S2 Relationships between Slatkin’s genetic distance among populations and the geographic distance between the lakes where the populations were sampled for Bulinus tropicus (multi-locus dataset, 15 lakes), Petasiger sp. (NAD1 marker, eight lakes) and Echinoparyphium sp. (NAD1 marker, four lakes). A linear regression line is fitted for illustration in the plot of B. tropicus as a near-significant isolation-by-distance pattern was observed using a Mantel test (P = 0.052). Geographic distances were log10-transformed in the B. tropicus plot to increase visual clarity.