Ability of short-chain fatty acids to reduce inflammation and attract leucocytes to the inflamed skin of gilthead seabream (Sparus aurata L.)

Abstract

The aim of the study was to investigate the potential preventive use of short-chain fatty acids (SCFAs) to modulate inflammatory responses in gilthead seabream (Sparus aurata) skin. Initially, in vitro experiments were conducted to evaluate the effects of various concentrations of butyric acid, acetic acid and propionic acid, as well as their combination, on the cytotoxicity and cell viability of three different cell lines. The results determined the safe concentration of SCFAs, which was then used for an in vivo study. Fish were allocated into six groups and administered different combinations of SCFAs via intramuscular injection, followed by an injection of carrageenan as an inflammatory agent. Skin samples were taken from the injection site three hours post-administration and used to analyse gene expression and immunohistochemistry. The results demonstrated that treatment with SCFAs resulted in increased expression of proinflammatory and anti-inflammatory genes and leucocyte markers in the inflamed skin of fish. The highest gene expression and recruitment of acidophilic granulocytes were observed in fish injected with propionic acid and carrageenan. It is concluded that acetic acid is the most effective anti-inflammatory SCFA tested in gilthead seabream exposed to acute inflammation induced by carrageenan injection. Acetic acid exhibited the most pronounced direct anti-inflammatory effect, although propionic acid appeared to play a significant role in several mechanisms contributing to the resolution of inflammation and recruitment of immune cells to the site of carrageenan-inflamed area in gilthead seabream skin.