article · Carbohydrate Polymer Technologies and Applications
A hybrid material combining microcrystalline cellulose and a calcium-based metal-organic framework has been developed for extracting the drug ritonavir from human plasma. The composite was synthesised using calcium chloride, soda water, 1,3,5-benzenetricarboxylic acid, and microcrystalline cellulose. Characterisation confirmed the successful incorporation of cellulose into the crystalline framework, which features a high surface area of 820 square metres per gramme. The hybrid was tested as a sorbent in dispersive solid-phase microextraction prior to high-performance liquid chromatography analysis. Method optimisation established that 12.50 milligrams of the hybrid sorbent and 250 microlitres of acetonitrile eluent provided the best extraction efficiency. By uniting the sustainability of microcrystalline cellulose with the large surface area of metal-organic frameworks, the composite demonstrated superior extraction performance compared to unmodified frameworks, indicating potential utility in biomedical sample preparation.
Accurately measuring therapeutic drugs in biological fluids requires efficient and sustainable extraction methods. Combining renewable microcrystalline cellulose with metal-organic frameworks creates a sorbent that offers both biodegradability and high surface area. This approach could assist biomedical and clinical laboratories in improving drug monitoring and sample purification while using smaller quantities of materials and solvents.
This technology provides an applied, laboratory-tested sample preparation method for biomedical analysis. Likely users include clinical testing laboratories and analytical facilities seeking efficient protocols for drug monitoring in blood plasma. The work represents early-stage method development validated on spiked plasma samples, meaning further validation on authentic patient samples and adaptation to standard commercial analytical workflows will be needed before it can be adopted in routine practice.
AI-generated from the published abstract. Always read the original work before citing.
A hybrid of microcrystalline cellulose (MCC) with metal organic frame (MOF) was developed as an efficient adsorbent for dispersive solid phase microextraction (dSPME) of ritonavir from spiked human plasma. The developed sorbent was characterized by a high-resolution scanning electron microscope (HR-SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The MCC was immersed in a solution of CaCl2, stirred with soda water and 1,3,5-benzenetricarboxylic acid, resulting in white MCC/MOF composites, which were separated, washed, and dried. The MCC/MOF hybrids were examined under the HR-SEM and showed morphological features different form the MCC and Ca-1,3,5-benzenetricarboxylic acid (Ca-BTC) MOF. The diffraction patterns of Ca-BTC/MCC composites clearly displayed the characteristic Ca-BTC MOF diffraction bands, indicating that MCC was successfully incorporated in the formation of crystalline MOF hybrids. The FTIR spectra exhibited the bands of MCC, as well as the bands of Ca-BTC MOFs. The prepared Ca-BTC/MCC MOF was applied for dSPME of ritonavir from human plasma, before the determination by high performance liquid chromatography with UV detection. After method optimization, the best extraction efficiency was achieved by using 12.50 mg of Ca-BTC/MCC MOF as a sorbent and 250 µL of acetonitrile as an eluent. This novel sorbent (Ca-BTC/MCC) merges the advantages of the high surface areas of MOFs 820 m2 g−1 with the biodegradability and sustainability of MCC. Besides, Ca-BTC/MCC exhibited higher extraction efficiency compared with unmodified Ca-BTC MOF. To the best of our knowledge, this work applies the Ca-BTC/MCC sorbents for dSPME of ritonavir in human plasma, for the first time. These results open the door to more applications of these composites in sample preparation for biomedical analysis.
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.1016/j.carpta.2024.100453
Is something wrong with this record? Report it or request removal.
Discussion
Have you built on this work, tried to replicate it, or seen it applied in practice? Share what you know. Verified researchers and MARATTO™ domain experts can open a discussion, and any member can reply. Contributions are reviewed before they appear.
No discussion yet. Open the first thread.
New to MARATTO™? Create a free account.
