article · ChemistryOpen
A carbon paste electrode modified with bentonite and L-cysteine enables the simultaneous detection and quantification of uric acid and ascorbic acid. Surface and compositional analysis of the electrode was conducted using electrochemical impedance spectroscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy. Electrochemical evaluations using cyclic voltammetry and square wave voltammetry demonstrated that the modified sensor provides good stability, sensitivity, selectivity, and regeneration capabilities. The sensor achieves limits of detection of 0.031 micromolar for uric acid and 9.6 micromolar for ascorbic acid, alongside linear response ranges of 0.1 to 100 micromolar and 10 to 1000 micromolar, respectively. A distinct peak-to-peak separation of 330 millivolts allows the two compounds to be distinguished without mutual interference. The device was successfully applied to monitor both acids in serum samples.
Uric acid and ascorbic acid frequently coexist in biological fluids and interfere with each other during electrochemical analysis. Developing a sensitive, selective, and regenerable sensor that cleanly differentiates between both compounds at low concentrations addresses a common analytical challenge, supporting more dependable monitoring of relevant physiological biomarkers in clinical and biochemical settings.
This sensing approach could benefit diagnostic equipment manufacturers and analytical laboratories seeking cost-effective, regenerable electrodes for fluid analysis. Judged by the abstract, this is early-stage, applied research tested on serum samples under laboratory conditions, meaning further device prototyping, operational lifetime testing, and regulatory validation are needed before real-world clinical use.
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Abstract A novel modification of a paste carbon electrode by Bentonite (Bent) and l ‐Cysteine ( l ‐Cyst) was carried out for uric acid (UA) and ascorbic acid (AA) detection and quantification. Morphological and compositional characterization of the electrode surface were carried out using electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and energy dispersive X‐ray spectroscopic analysis (EDS). Cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were used to analyze UA and AA. The obtained sensor shows a good stability, sensibility, selectivity, and regeneration ability. Accordingly, the limit of detection (LOD) is found to be 0.031 μ m and 9.6 μ m for UA and AA, respectively. A good linearity in the range of 0.1 to 100 μ m for UA and 10 to 1000 μ m for AA was obtained. The peak‐to‐peak separation of UA‐AA (Δ E UA‐AA ) was determined to be 330 mV. In addition, the sensor is applied successfully to monitor UA and AA in serum samples.
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DOI: 10.1002/open.202200201
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